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KMID : 0381120220440080957
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Genes and Genomics
2022 Volume.44 No. 8 p.957 ~ p.966
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Gene expression profiling and in vitro functional studies reveal RAD54L as a potential therapeutic target in multiple myeloma
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Bong Ivyna Pau Ni
Ng Ching Ching
Othman Norodiyah
Esa Ezalia
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Abstract
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Background: Current advances in the molecular biology of multiple myeloma (MM) are not sufficient to fully delineate the genesis and development of this disease.
Objective: This study aimed to identify molecular targets underlying MM pathogenesis.
Methods: mRNA expression profiling for 29 samples (19 MM samples, 7 MM cell lines and 3 controls) were obtained using microarray. We evaluated the in vitro effects of RAD54L gene silencing on the proliferation, apoptosis and cell cycle distribution in KMS-28BM human MM cells using siRNA approach. Cell proliferation was determined by MTS assay while apoptosis and cell cycle distribution were analysed with flow cytometry. Gene and protein expression was evaluated using RT-qPCR and ELISA, respectively.
Results: Microarray results revealed a total of 5124 differentially expressed genes (DEGs), in which 2696 and 2428 genes were up-regulated and down-regulated in MM compared to the normal controls, respectively (fold change?≥?2.0; P?
Conclusions: This study has identified possible molecular targets underlying the pathogenesis of MM. For the first time, we reveal RAD54L as a potential therapeutic target in MM, possibly functioning in the cell cycle and checkpoint control.
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KEYWORD
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Multiple myeloma, Differentially expressed genes, RAD54L, Cell proliferation, Apoptosis, Cell cycle
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